概述

产品名称：PE Anti-Mouse F4/80 Monoclonal Antibody

描述：F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8 T cells-mediated peripheral tolerance.

应用范围：FCM

种属反应性：Mouse

免疫原：Rat

性能

形态：liquid

存储条件：Keep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.

存储溶液：PBS pH 7.2, 0.09% NaN3, 0.2% BSA

纯度：0.2 mg/ml

克隆：BM8

亚型：Rat IgG2a

应用

应用说明：Recommended Application: FC

功能：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used ≤ 1.0 µg /10^6 cells in 100 µl). Since applications vary, the appropriate dilutions must be determined for individual use.

图像

PE Anti-Mouse F4-80 Monoclonal Antibody.jpg

BALB/C bone marrow cells were cultured with GMCSF for 2 weeks then stained with PE anti-mouse F4/80 antibody.