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β-Actin-HRP mouse monoclonal antibody
β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues
WB
Human, Rat, Mouse, Fruit fly
Full-length β-Actin protein of human
Liquid
Store at -20°C or below. Avoid repeated freezing and thawing cycles
1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Mouse IgG
WB: 1:1000~1:10000 Optimal dilutions /concentrations should be determined by the end user
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