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Biotin Anti-Human CD3 (HIT3a) Monoclonal Antibody
The HIT3a monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits γ and δ, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR with HIT3a initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.
FCM
Human
Mouse
liquid
Keep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
HIT3a
Mouse IgG2a
Recommended Application: FC
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used ≤0.5 µg per million cells in 100 µl volume. Please check your vial). Since applications vary, the appropriate dilutions must be determined for individual use.
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