iFluor is a totally new series of fluorescent dyes. Compared with traditional Cy series of fluorescent dye, it has incomparable advantages in brightness, and stability, etc. In the market, it can be equivalent even beyond usual series of Alexa Fluor dyes in some respects. The conjugates formed by these dyes exclusively exhibit the most excellent optical property. In addition, this series of dyes can conjugate with R-PE or APC, and subsequently generate Tandem dyes, which expands the application of iFluor in multicolor detection.
iFluor advantages:
• Brightness —— iFluor conjugates show stronger fluorescent comparing with other dyes.
• Light stability —— iFluor conjugates demonstrate stronger light stability comparing with other dyes, and they also provide longer time of image capturing.
• Instrument compatibility —— for the absorption spectrum of iFluor conjugates, it matches well with main output wavelength of ordinary excitation light.
• A variety of colors —— iFluor conjugates have multiple kinds of direct fluorescence, which are ranged from blue to red.
• insensitive pH value —— iFluor dyes maintain strong fluorescence in a wide pH range.
• Water-soluble —— iFluor reactive dyes have good solubility in water, they can bind with proteins without organic reagents, and they are not easy to produce precipitation in storage.
Corresponding form of iFluor products to common phthalocyanine fluorescent dyes
iFluor products
|
Corresponding fluorescent dyes of common phthalocyanine
|
Ex/Em
|
iFlour 488
|
Cy2,FITC
|
498/529
|
iFlour 555
|
Cy3,TRITC (Rhodamine)
|
556/572
|
iFlour 647
|
Cy5
|
651/668
|
iFlour 680
|
Cy5.5
|
685/704
|
iFlour 750
|
Cy7
|
759/782
|
Excitation and absorption spectrum of iFluor products